Journal article
Enterocytozoon bieneusi genotypes in people with gastrointestinal disorders in Queensland and Western Australia
Y Zhang, AV Koehler, T Wang, GJ Robertson, RS Bradbury, RB Gasser
Infection Genetics and Evolution | ELSEVIER | Published : 2018
Abstract
Enterocytozoon bieneusi is the commonest pathogenic microsporidian found in humans and animals in many countries, but there is scant information on this pathogen in Australia. Here, we conducted the first molecular epidemiological investigation of E. bieneusi in humans with gastrointestinal disorders in Queensland and Western Australia. Genomic DNAs derived from 605 individual faecal samples from children (n = 279) and adults (n = 326) were extracted, and then subjected to nested PCR-based sequencing of the internal transcribed spacer (ITS) of nuclear ribosomal DNA to detect and characterise E. bieneusi. Enterocytozoon bieneusi was detected in eight of 605 human faecal samples (1.3%), includ..
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Awarded by Australian Research Council
Funding Acknowledgements
Research funding from the Australian Research Council (grant number LP160101299) and Melbourne Water Corporation (RBG and AVK) is gratefully acknowledged. Yan Zhang was a recipient of scholarships from the Chinese Scholarship Council (CSC) and The University of Melbourne. The authors thank colleagues from Townsville Hospital Health Services for providing samples, and Constantine E. Gasser for comments on statistics. The use of samples was approved by the Human Research Ethics Committees of Townsville Hospital and Health Services (reference no. HREC/14/QTHS/4), and by the University of Tasmania (reference no. H11549).